LOCUS       ATH1L-pGEc2             7437 bp    DNA     circular     21-SEP-2016
FEATURES             Location/Qualifiers
     misc_feature    complement(6915..7044)
                     /note="3'-ITR"
     misc_feature    complement(6613..6827)
                     /note="BGH pA"
     rep_origin      1137..1804
                     /dnas_title="Col E1 origin"
                     /label=Col E1 origin
                     /vntifkey="33"
     CDS             129..989
                     /dnas_title="AmpR"
                     /label=AmpR
                     /vntifkey="4"
     misc_feature    1998..2127
                     /note="5'ITR"
     misc_feature    2208..2790
                     /note="CMV Promoter"
     misc_feature    2828..2960
                     /note="Artificial Intron"
     misc_feature    6065..6606
                     /note="WPRE"
     misc_recomb     complement(2974..3001)
                     /label=attB1
                     /note="attB1 site"
                     /note="recognized as attB1"
     misc_feature    5244..5266
                     /note="attB2"
     misc_feature    6044..6049
                     /note="BstB1 site"
     misc_feature    5267..5962
                     /note="Super GFP"
     misc_feature    5963..6013
                     /note="AviTag"
     misc_feature    6014..6037
                     /note="His Tag"
     PCR_frame       3011..5243
                     /note="Reading Frame"
                     /set="Set 1"
     misc_feature    3011..5221
                     /note="OPTIMIZED ATH1L coding region"
     misc_feature    5222..5243
                     /note="TEV"
     vector          join(5252..7437,1..2991)
                     /source="pGEc2-DEST"
                     /type="Destination Vector"
     clone           2992..5251
                     /source="ATH1L pDONR"
                     /type="Entry Clone"
     source          1..7437
                     /dnas_title="ATH1L-pGEc2"
ORIGIN      
        1 GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT
       61 CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA
      121 GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT
      181 GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT
      241 TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT
      301 TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG
      361 TATTATCCCG TATTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA
      421 ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA
      481 GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA
      541 CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA
      601 CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA
      661 CCACGATGCC TGTAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA
      721 CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC
      781 TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC
      841 GTGGTTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG
      901 TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA
      961 TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT
     1021 AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA
     1081 ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG
     1141 AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA
     1201 CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT
     1261 TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTTCTT CTAGTGTAGC
     1321 CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA
     1381 TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA
     1441 GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC
     1501 CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA
     1561 GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA
     1621 CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG
     1681 GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC
     1741 TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG
     1801 CTCATTAGGC ACCCCAGGCT TTACCCGAAC GACCGAGCGC AGCGAGTCAG TGAGCGAGGA
     1861 AGCGGAGAGC GCCCAATACG CAAGGAAACA GCTATGACCA TGTTAATGCA GCTGGCACGA
     1921 CAGGTTTCCC GACTGGAAAG CGGGCAGTGA GCGGAAGGCC CATGAGGCCA GTTAATTAAG
     1981 AGGTACCTTA ATTAAGGCTG CGCGCTCGCT CGCTCACTGA GGCCGCCCGG GCAAAGCCCG
     2041 GGCGTCGGGC GACCTTTGGT CGCCCGGCCT CAGTGAGCGA GCGAGCGCGC AGAGAGGGAG
     2101 TGGCCAACTC CATCACTAGG GGTTCCTTGT AGTTAATGAT TAACCCGCCA TGCTACTTAT
     2161 CTACGTAGCC ATGCTCTAGG AAGATCGCAA TTCGCCCTTA AGCTAGCTAG TTATTAATAG
     2221 TAATCAATTA CGGGGTCATT AGTTCATAGC CCATATATGG AGTTCCGCGT TACATAACTT
     2281 ACGGTAAATG GCCCGCCTGG CTGACCGCCC AACGACCCCC GCCCATTGAC GTCAATAATG
     2341 ACGTATGTTC CCATAGTAAC GCCAATAGGG ACTTTCCATT GACGTCAATG GGTGGAGTAT
     2401 TTACGGTAAA CTGCCCACTT GGCAGTACAT CAAGTGTATC ATATGCCAAG TACGCCCCCT
     2461 ATTGACGTCA ATGACGGTAA ATGGCCCGCC TGGCATTATG CCCAGTACAT GACCTTATGG
     2521 GACTTTCCTA CTTGGCAGTA CATCTACGTA TTAGTCATCG CTATTACCAT GGTGATGCGG
     2581 TTTTGGCAGT ACATCAATGG GCGTGGATAG CGGTTTGACT CACGGGGATT TCCAAGTCTC
     2641 CACCCCATTG ACGTCAATGG GAGTTTGTTT TGGCACCAAA ATCAACGGGA CTTTCCAAAA
     2701 TGTCGTAACA ACTCCGCCCC ATTGACGCAA ATGGGCGGTA GGCGTGTACG GTGGGAGGTC
     2761 TATATAAGCA GAGCTGGTTT AGTGAACCGT CAGATCCTGC AGAAGTTGGT CGTGAGGCAC
     2821 TGGGCAGGTA AGTATCAAGG TTACAAGACA GGTTTAAGGA GACCAATAGA AACTGGGCTT
     2881 GTCGAGACAG AGAAGACTCT TGCGTTTCTG ATAGGCACCT ATTGGTCTTA CTGACATCCA
     2941 CTTTGCCTTT CTCTCCACAG GTGTCCAGCG GCCATCACAA GTTTGTACAA AAAAGCAGGC
     3001 TCGCGCCACC ATGGAAGATG CCGGCGAGGA TCCTACAACA TTTGCCGCCC ATAGCCTGCC
     3061 CAGCGATCCT AGACTGCTGG CCACAGTGAC AAATGCCTAC CTGGGCACCA GAGTGTTCCA
     3121 CGATACACTG CATGTGTCCG GCGTGTACAA TGGCGCTGGC GGAGATACAC ATAGAGCCAT
     3181 GCTGCCTAGC CCCCTGAATG TGCGACTGGA AGCTCCTGCT GGAATGGGCG AGCAGCTGAC
     3241 AGAAACCTTC GCCCTGGATA CCAATACCGG CAGCTTCCTG CACACCCTGG AAGGCCCTAG
     3301 ATTCAGAGCC AGCCAGTGCA TCTACGCCCA CAGAACACTG CCTCACGTGC TGGCCTTTAG
     3361 AGTGTCTATT GCCAGACTGG CCCCTGGCAG CGGACCTATT ACACTGCTGC TGAGAAGCGC
     3421 CTTCAGCCCC GAGTCTCCTG ATCTGGATCT GCACCAGGGC CCAGATTTCC AGGGGGCCAG
     3481 ATACCTGTAT GGCCACACAC TGACACCTGA GCAGCCTGGT GGACCTCAGC AGGAAGTGCA
     3541 TATGCTGTGG ACACCAGCCC CTCCAGATCT GACACTGGGA GAAGGCGAAG AAGCCAGAAC
     3601 CTGGGATTTT CTGACAGCCG TGGGAGGATC TCAGGCCGAA GCTCAGGCTT GTCTGACTGA
     3661 AGCACTGCAG CTCCAGGCCA GAGGCGCTCT GTATACTGCT CATGCTCAGG CATGGGCCCA
     3721 GCTGTGGGTG GAATGTGGAC TCGATGTCGT GGGACCTCTG CAGCTGAGAC AGGCTCTGAG
     3781 AGGCAGCCTG TACTATCTGC TGTCTGCCCT GCCTCAGCCT AAGGCCCCAG GATATATCTG
     3841 TCACGGACTG AGCCCTGGCG GCCTGTCTAA TGGAAGCAGA GAGGAATGTT ACTGGGGCCA
     3901 CGTGTTCTGG GACCAGGACC TGTGGATGTT CCCCAGCATC CTGATGTTTC ACCCTGAGGC
     3961 TGCCAGAGCC ATCCTGGAAT ATCGGATCAG AACTCTGGAC GGCGCCCTCG AGAATGCCCA
     4021 GAATCTCGGA TATCAGGGCG CCAAGTTTGC CTGGGAGTCT GCCGATTCAG GACTCGAAGT
     4081 GTGCCCTGAG GATATCTACG GCGTCCAGGA AGTCCACGTC AACGGCGCTG TGGTGCTGGC
     4141 TTTTGAGCTG TACTACCACA CCACCCAGGA TCTCCAGCTG TTTAGAGAAG CCGGTGGCTG
     4201 GGATGTCGTC AGAGCCGTGG CTGAGTTTTG GTGCTCCAGA GTGGAATGGT CCCCCAGAGA
     4261 AGAGAAGTAC CACCTGAGGG GCGTGATGAG CCCAGATGAG TATCACAGCG GCGTGAACAA
     4321 CTCCGTGTAC ACCAACGTGC TCGTGCAGAA CTCCCTGAGA TTTGCTGCCG CTCTCGCACA
     4381 GGATCTGGGA CTGCCTATTC CATCTCAGTG GCTGGCCGTG GCCGATAAGA TCAAGGTGCC
     4441 CTTCGATGTG GAACAGAACT TCCACCCCGA GTTCGATGGC TATGAGCCTG GCGAAGTGGT
     4501 CAAGCAGGCA GATGTGGTCC TCCTGGGCTA CCCTGTGCCA TTTTCCCTGT CTCCCGACGT
     4561 GCGGAGAAAG AACCTCGAGA TCTATGAGGC CGTGACCTCT CCACAGGGAC CTGCCATGAC
     4621 TTGGTCTATG TTTGCCGTGG GCTGGATGGA ACTGAAGGAT GCAGTCAGAG CTAGAGGGCT
     4681 GCTGGACAGA TCCTTCGCCA ATATGGCCGA GCCCTTCAAA GTGTGGACAG AGAACGCTGA
     4741 TGGATCTGGC GCCGTGAATT TCCTGACCGG CATGGGAGGT TTTCTGCAGG CAGTGGTGTT
     4801 CGGCTGCACA GGCTTCAGAG TGACAAGAGC CGGCGTGACC TTCGATCCAG TGTGTCTGTC
     4861 TGGCATCAGC AGGGTGTCCG TGTCCGGAAT CTTCTACCAG GGCAACAAGC TGAACTTTAG
     4921 CTTCAGCGAG GACAGCGTGA CCGTGGAAGT GACAGCTAGA GCTGGACCTT GGGCTCCTCA
     4981 TCTCGAAGCT GAGCTGTGGC CTAGCCAGAG CAGACTGTCT CTGCTCCCTG GACACAAGGT
     5041 GTCATTCCCT AGATCCGCCG GCAGAATTCA GATGTCCCCT CCAAAGCTGC CTGGCTCCTC
     5101 TAGCAGCGAG TTTCCTGGCA GAACCTTCTC CGATGTGCGG GATCCTCTCC AGTCTCCTCT
     5161 GTGGGTCACA CTCGGATCTT CTAGCCCTAC CGAGAGCCTG ACAGTGGACC CTGCTTCTGA
     5221 Agaaaacttg tactttcaag gctCCAGCTT TCTTGTACAA AGTGGTATGA GCAAGGGCGA
     5281 GGAACTGTTC ACCGGCGTGG TGCCCATCCT GGTGGAACTG GATGGCGACG TGAACGGCCA
     5341 CAAGTTCTCT GTGCGGGGAG AGGGCGAAGG CGACGCCACA AATGGCAAGC TGACCCTGAA
     5401 GTTCATCTGC ACCACCGGCA AGCTGCCCGT GCCTTGGCCT ACCCTCGTGA CCACACTGAC
     5461 CTACGGCGTG CAGTGCTTCA GCAGATACCC CGACCATATG AAGCGGCACG ACTTCTTCAA
     5521 GAGCGCCATG CCCGAGGGCT ACGTGCAGGA ACGGACCATC AGCTTCAAGG ACGACGGCAC
     5581 CTACAAGACC AGAGCCGAAG TGAAGTTCGA GGGCGACACC CTCGTGAACC GGATCGAGCT
     5641 GAAGGGCATC GATTTCAAAG AGGACGGCAA CATCCTGGGC CACAAGCTGG AGTACAACTT
     5701 CAACAGCCAC AACGTGTACA TCACCGCCGA CAAGCAGAAG AACGGCATCA AGGCCAACTT
     5761 CAAGATCCGG CACAACGTGG AAGATGGCAG CGTGCAGCTG GCCGACCACT ACCAGCAGAA
     5821 CACCCCCATC GGAGATGGCC CCGTGCTGCT GCCCGACAAC CACTACCTGA GCACCCAGAG
     5881 CGTGCTGAGC AAGGACCCCA ACGAGAAGCG GGACCACATG GTGCTGCTGG AATTCGTGAC
     5941 CGCCGCTGGC ATCACCCACG GCATGAGCGG CCTGAACGAC ATCTTCGAGG CCCAGAAAAT
     6001 CGAGTGGCAC GAGCACCACC ATCATCACCA TCACCACTGA TGATTCGAAA AATAATAAGG
     6061 ATCCAATCAA CCTCTGGATT ACAAAATTTG TGAAAGATTG ACTGGTATTC TTAACTATGT
     6121 TGCTCCTTTT ACGCTATGTG GATACGCTGC TTTAATGCCT TTGTATCATG CTATTGCTTC
     6181 CCGTATGGCT TTCATTTTCT CCTCCTTGTA TAAATCCTGG TTGCTGTCTC TTTATGAGGA
     6241 GTTGTGGCCC GTTGTCAGGC AACGTGGCGT GGTGTGCACT GTGTTTGCTG ACGCAACCCC
     6301 CACTGGTTGG GGCATTGCCA CCACCTGTCA GCTCCTTTCC GGGACTTTCG CTTTCCCCCT
     6361 CCCTATTGCC ACGGCGGAAC TCATCGCCGC CTGCCTTGCC CGCTGCTGGA CAGGGGCTCG
     6421 GCTGTTGGGC ACTGACAATT CCGTGGTGTT GTCGGGGAAG CTGACGTCCT TTCCATGGCT
     6481 GCTCGCCTGT GTTGCCACCT GGATTCTGCG CGGGACGTCC TTCTGCTACG TCCCTTCGGC
     6541 CCTCAATCCA GCGGACCTTC CTTCCCGCGG CCTGCTGCCG GCTCTGCGGC CTCTTCCGCG
     6601 TCTTCGAGAT CTGCCTCGAC TGTGCCTTCT AGTTGCCAGC CATCTGTTGT TTGCCCCTCC
     6661 CCCGTGCCTT CCTTGACCCT GGAAGGTGCC ACTCCCACTG TCCTTTCCTA ATAAAATGAG
     6721 GAAATTGCAT CGCATTGTCT GAGTAGGTGT CATTCTATTC TGGGGGGTGG GGTGGGGCAG
     6781 GACAGCAAGG GGGAGGATTG GGAAGACAAT AGCAGGCATG CTGGGGACTC GAGTTAAGGG
     6841 CGAATTGCCG ATTAGGATCT TCCTAGAGCA TGGCTACGTA GATAAGTAGC ATGGCGGGTT
     6901 AATCATTAAC TACAAGGAAC CCCTAGTGAT GGAGTTGGCC ACTCCCTCTC TGCGCGCTCG
     6961 CTCGCTCACT GAGGCCGGGC GACCAAAGGT CGCCCGACGC CCGGGCTTTG CCCGGGCGGC
     7021 CTCAGTGAGC GAGCGAGCGC GCAGCCTTAA TTAAGAGCTC ATGGCGCGCC TAGGCCTTGA
     7081 CGGCCTTCCG CCAATTCGCC CTATAGTGAG TCGTATTACG TCGCGCTCAC TGGCCGTCGT
     7141 TTTACAACGT CGTGACTGGG AAAACCCTGG CGTTACCCAA CTTAATCGCC TTGCAGCACA
     7201 TCCCCCTTTC GCCAGCTGGC GTAATAGCGA AGAGGCCCGC ACCGAAACGC CCTTCCCAAC
     7261 AGTTGCGCAG CCTGAATGGC GAATGGGAGC GCCCTGTAGC GGCCACTCAA CCCTATCTCG
     7321 GTCTATTCTT TTGATTTATA AGGGATTTTG CCGATTTCGG CCTATTGGTT AAAAAATGAG
     7381 CTGATTTAAC AAAAATTTAA CGCGAATTTT AACAAAATAT TAACGCTTAC AATTTAG
//

